This widely used immunoassay to probe samples for specific proteins and posttranslational modifications has potential time and material savings associated with variations of the transfer step. Whether it’s a simple buffer switch, or a more involved equipment swap - it’s always worthwhile to explore lab workflow efficiencies. Just take care not to risk a successful blot!
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Coomassie Blue SDS-PAGE Gel Trends: A conventional dye goes radical
Coomassie blue protein gel staining began in the 1960s, and it’s still a fan favorite in biology labs today. While less sensitive as a colormetric method than silver, or fluorescent staining, Coomassie has undergone a significant revolution in recent years. This post presents a few handy tips for this essential life science pigment.
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